ABSTRACT
Objective: To study the induction of human MCF-7 breast cancer cell apoptosis by inotodiol isolated from Inonotus obliquus, a traditional Chinese medicinal fungi. Methods: Inotodiol was isolated from the crude ethanol extract of I. obliquus and purified. Inotodiol at different concentration and human breast cancer MCF-7 cell line were co-incubated. Relative cell viabilities were determined by MTT method; apoptosis was analyzed by Annexin V/PI dual staining with flow cytometry; The protein expression levels of Caspase-3 and Poly ADP ribose polymerase (PARP) were detected by Western blotting. Results: Inotodiol reduced the viability of MCF-7 cells dose-and time-dependently. Moreover, inotodiol induced the apoptosis in MCF-7 cells. The data of Western blotting showed that inotodiol up-regulated the expression levels of cleaved Caspase-3 and PARP, in a time-dependent manner. Conclusion: Inotodiol could induce apoptosis in human breast cancer cells, which provides novel information for clinical application of inotodiol and its preparations for the breast cancer patients.
ABSTRACT
Objective: To study the triterpenoids from the fruiting body of Inonotus obliquus. Methods: The compounds were isolated and purified by silica gel, MCI gel, Sephadex LH-20 column chromatographies, and their structures were mainly elucidated on the basis of physicochemical characteristics and spectral analysis. Results: Fifteen compounds were isolated from 95% EtOH extract of I. obliquus, and were identified as 3-oxo-lanosta-8, 24-diene-21-al (1), lanosterol (2), 3β-hydroxy-lanosta-8, 24-diene-21-al (3), betulin (4), inotodiol (5), trametenolic acid (6), 3β, 21-dihydroxy-lanosta-8, 24-diene (7), oleanic acid (8), ursolic acid (9), betulinic acid (10), inonotusane A (11), inoterpene D (12), 3-O-acetyl-11α, 12α-epoxy-oleanan-28, 13β-olide (13), ergosterol (14), and ergosta-4, 6, 8, 22-tetraene-3-one (15), respectively. Conclusion: Compound 1 is a new triterpenoid named as inonotusane D, while compounds 9, 13, and 15 are isolated from I. obliquus for the first time.